Detection of the mycotoxin Zearalenone from the filtrate of Fusarium oxysporum isolated from onions

Abstract

In this research, samples of onions were collected from several areas in the city of Misurata,represented in Al-Dafniyah, Tomina, Zawiat Al-Mahjoub, Al-Ghiran, Quzir, Al-Jazeera, Misurata Markaz, Ras Al-Toba, Al-Zarrouk and Qasr Ahmed, during the period from June to November 2018. Sampling, which was represented in a kilo of onions from each region, where the number of complete samples was 50 samples, and for each sample the Fusarium oxysporum grew 3 replicates, where the number of dishes was 150 dishes, then the F.oxysporum was isolated From the basal part of the onion plant (discoid stems and fibrous roots) that showed symptoms of the disease, and then grown on solid nutrient medium Potato dextrose agar (PDA) and then grown in test tubes containing liquid Nutrient broth (NB) at 28 ±2”C in order to measure the wet and dry weight of the filter and also for the purpose of detecting the zearalenone toxin from the filtrate. The process of detecting the mycotoxin zearalenone was carried out in two steps. First, the sample was prepared and added 100 μ l of the Bufffer comes with the kit for the test on 600 μ l of the filtrate in special tubes with a cap with simple shaking to ensure the homogeneity of the solution together, Secondly, injection with special materials consisting of a standard solution. enzyme conjugate, substrate, chromogen and stop solution, and zearalenone readings were taken by ELISA READER device, where it was proved through this research that F. oxysporum is one of the fungi that secrete zearalenone toxin.