Histopathological Assessment of the Effects of Ziziphus spina-christi Leaf Extract on Renal and Hepatic Tissues in Male Rabbits

Sana Mohammed; EDA ALSHAILABI; Asma  Ahmed; Fayrouz  Khaled

doi:10.36602/jsba.2026.21.104

Authors

Sana A. A. Mohammed Pathology and Clinical Pathology Department, Faculty of Veterinary Medicine, Omar Al-Mukhtar University, Libya.
EDA ALSHAILABI OMAR ALMUKHTAR UNIVERSITY
Asma I. Ahmed Libyan Academy for Postgraduate Studies, Biomedicine Department, Jabal Al-Akhdar Branch, Libya
Fayrouz A. Khaled Chemistry Department, Omar Al-Mukhtar University, Faculty of Science, Libya.

DOI:

https://doi.org/10.36602/jsba.2026.21.104

Abstract

The present study aimed to evaluate the histopathological effects of Ziziphus spina‑christi leaf extract (ZSCLE) on liver and kidney tissues in male rabbits. Ten adult rabbits were divided into two groups: a control group receiving no treatment and a treatment group administered ZSCLE orally at a dose of 200 mg/kg/b.w. for six weeks. Histopathological examination of liver tissues in the treated group revealed dilated and congested central veins, portal area degeneration, inflammatory cell infiltration, fibrosis, and hemorrhage. Likewise, kidney sections exhibited glomerular degeneration, tubular necrosis, desquamation of tubular epithelium, damaged brush borders, and interstitial hemorrhage. These findings demonstrate that prolonged oral administration of ZSCLE at the 200 mg/kg/b.w. doses can induce substantial structural alterations in these organs, despite their reported antioxidant and anti-inflammatory properties. Further studies are recommended to determine safe therapeutic doses, evaluate biochemical markers, and elucidate the underlying mechanisms of action for these organs and other organs.

Author Biography

Sana A. A. Mohammed, Pathology and Clinical Pathology Department, Faculty of Veterinary Medicine, Omar Al-Mukhtar University, Libya.

قسم علم الأمراض وعلم الأمراض السريري، كلية الطب البيطري، جامعة عمر المختار، ليبيا.

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